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Flow cytometry - continuation course

Aim and format

The course aims to provide a broad understanding of different aspects of flow cytometry, including experimental design, sample material handling, analysis of flow cytometry data, as well as file and image handling prior to research publication. The course provides practical knowledge of advanced flow cytometry that can be directly transferred to research questions.

Learning outcomes

On completion of the course, the participants shall be able to:

  • set up FACS analysis instruments and conduct quality controls

  • analyse flow cytometry data

  • formulate a topical research question and select relevant method(s) within the area of flow cytometry.

Course content

The course includes the following elements:

  • the theory behind flow cytometry

  • applications of flow cytometry within biomedical research

  • sources of error, quality assurance, validation of flow cytometry methods

  • FACS experiments: planning, implementation and analysis of flow cytometry data.

Couse design

The course comprises theoretical lectures associated with advanced flow cytometry, practical demonstrations, theoretical and practical group work, as well as individual discussion forums and practical exercises regarding specific research questions in which advanced flow cytometry is applied. The results of these exercises are presented to, and discussed by, the course coordinator and course participants.


  • Anna Fossum, FACS Core Facility at Lund Stem Cell Center
  • Anna Hammarberg, MultiPark - Cellomics and Flow Cytometry Core Facility
  • Charlotta Böiers, FACS Core Facility at Lund Stem Cell Center
  • Zhi Ma, FACS Core Facility at Lund Stem Cell Center


  • Completion of basic training in flow cytometry (Basic course in flow cytometry or equivalent)
  • Active FACS user

Course fee

  • RSSCB and Multipark members: No course fee
  • External participants: 4000 SEK

This course is a co-arrangement between the Research School in Stem Cell Biology, the Lund University Neuroscience Graduate School, the StemTherapy FACS Core Facility at Lund Stem Cell Center, and the MultiPark Cellomics and Flow Cytometry Core Facility. Please be aware that the number of participants is limited. Preference is given to PhD students and postdoctoral fellows who are affiliated with these networks, and to active FACS users.


Page Manager:

When and Where?

May 11-15, 2020

Time and venue: TBD

Applications will open in February 2020.


Syllabus MEFLFO1 

Flow Cytometry - continuation course, MEFLFO1 

1.5 credits, Third cycle

Flow Cytometry, continuation course (pdf 124,9 kB)


Charlotta Böiers
Operational director StemTherapy FACS Core Facility
Charlotta [dot] Boiers [at] med [dot] lu [dot] se

Christine Karlsson
Executive director Research School in Stem Cell Biology
Christine [dot] Karlsson [at] med [dot] lu [dot] se

Joan Yuan
Scientific director StemTherapy FACS Core Facility
Joan [dot] Yuan [at] med [dot] lu [dot] se