Lund Stem Cell Center Imaging Facility
The Imaging Core Facility offers microscopy systems that enable advanced imaging of fluorescent and non-fluorescent live cells and fixed-stained biological specimens.
Instruments available at the Core include:
Olympus VS-120 Virtual Slide Microscope
The Olympus VS-120 Virtual Slide Microscope allows for fully automated high-speed and high-resolution bright field and multi-fluorescence digital images of entire histological slides or selected areas within them. The system is equipped with 2x, 4x, 10x, 20x and 40x objectives and DAPI, FITC, Cy3, Cy5, Cy7 high-speed filters.
The Olympus VS-120 can scan multiple large specimens to 31 Z-planes that allow for the acquisition of multi-plane virtual slides at different magnification, and provides with accurate imaging stitching of composite images across large specimen regions.
Zeiss 780 Confocal Laser-Scanning Inverted Microscope
The Zeiss 780 confocal laser-scanning inverted microscope allows for high-resolution imaging of fixed unstained and stained fluorescent samples and time-lapse imaging of live-cells. The Zeiss 780 is equipped with six laser lines 405 nm, 458/488/514 nm, 561 nm and 633 nm and 10x, 20x, 40x water, 40x oil, 63x water, 63x oil objectives.
The instrument employs the spectral detector GaAsp that allows for high sensitivity detection of 32-channels and three additional detectors (PMTs), two for confocal detection and one for transmitted light detection. The Zeiss 780 has a motorized stage and an incubation chamber that allows for temperature, humidity, and CO2 control during time-lapse live cells experiments.
Leica Stellaris 5 Confocal Laser-Scanning Inverted Microscope
The Leica Stellaris 5 confocal laser-scanninginverted microscope allows for high-resolution imaging of fixed unstained and stained fluorescent samples and time-lapse imaging of live-cells/tissue sections.The Leica Stellaris 5 is equipped with a White LightLaser (WLL) as excitation light source which converts monochromatic laser light into a rainbow of spatially coherent light covering a large part of the visible spectrum. From this “laser rainbow” any wavelength and intensity of light can be selected to excite a sample. The system is equipped with Hybrid detectors, Power HyD S,which enable sensitive detection of fluorescence and a resonant scanner for fast image acquisition and detection of rapidly occurring processes, especially in living samples.
The TauSensetechnology supplied by the Stellaris 5 confocal system is a set of imaging tools based on fluorescence lifetime, which gives access to functional imaging, allows to remove undesired signal contribution and enables to separate spectrally overlapping fluorophores. The following objectives are available: 5x, 10x, 20xmulti-immersion water/glycerine/oil, 40x glycerol, 40x oil, 63x glycerol, and 63x oil.
*The acquisition of the Leica Stellaris 5 is a joint effort between StemTherapy group leader Paul Bourgine and the StemTherapy Imaging Facility.