FACS Instruments
Learn more about the cell sorters and analysers available at the FACS Core Facility
Sorters:
BD FACS Aria III
The BD FACS Aria III has 5 laser lines: near UV at 375nm, violet at 405nm, blue at 488nm, yellow-green at 561 nm and red at 635nm. The violet and near UV are co-linear and cannot be used simultaneously. It can detect up to 16 colours and two scatter parameters.
Configuration Protocol: BD FACS Aria III Filters and Mirrors
Plate Set-Up Protocol: BD FACS Aria III Plate Set-Up Protocol
BD FACS Aria IIu SORP
The Aria IIu SORP cell sorter has 3 laser lines: violet at 405nm, blue at 488nm and red at 635nm. It can detect up to 12 colours and two scatter parameters.
Configuration Protocol: BD FACS Aria IIu SORP Filters and Mirrors
Plate Set-Up Protocol: BD FACS Aria IIu SORP Plate Set-Up Protocol
BD FACS Melody
The BD FACS Melody is contained within a biosafety hood which makes it suitable for sorting primary human samples and transduced cells. The Melody has three laser lines: violet at 405 nm, blue at 488 nm and yellow-green at 561 nm. It can detect 8 colours and two scatter parameters.
Configuration Protocol: BD FACS Melody Filters and Mirrors
BD FACSymphony S6
Our most advanced and latest addition! The BD FACSymphony S6 has 5 laser lines: UV at 355nm, violet at 405nm, blue at 488nm, yellow-green at 561 nm and red at 635nm. It can detect 30 colours and two scatter parameters.
Configuration Protocol: BD FACS Symphony s6 Cell Sorter Filter Configuration
Analysers:
BD Symphony A1
The BD Symphony A1 has four laser lines: violet at 405 nm, blue at 488 nm, yellow-green at 561 nm and red at 637 nm. It can detect 16 colours, two scatter parameters and also has a small particle side scatter detector.
Configuration Protocol: BD Symphony A1 Filter Configuration
BD LSR Fortessa
The BD LSR Fortessa has four laser lines: violet at 405 nm, blue at 488nm, yellow-green at 561 nm and red at 640 nm. It can detect 17 colours and two scatter parameters.
Configuration Protocol: BD FACS LSR Fortessa SORP Filters and Mirrors
BD LSR Fortessa X20
This analyser is a good supplement to our BD LSRFortessa and also has the UV laser to give more fluorochrome choices for your multi-colour analysis. The X-20 has five laser lines: UV at 375 nm, violet at 405 nm, blue at 488nm, yellow-green at 561 nm and red at 640 nm. It can detect 18 colours and two scatter parameters.
Configuration Protocol: BD LSR Fortessa X20 Filters and Mirrors
