ATAC-seq and ChIP-seq
This workshop aims to provide understanding into ChIP- and ATAC-sequencing. Briefly, the workshop recapitulates the bench workflows, how to prepare your data, call peaks, annotation of peaks in the genome, motif analysis and differential peak analysis. Differences between ChIP-and ATAC-sequencing will be highlighted.
- Quick repetition of the workflow/theory of the technique
- Differences between ChIP-seq and ATAC-seq
- Preprocessing (mapping/dealing with genomic repeats etc./removal of low quality alignments)
- Peak calling (Homer/MACS2) – narrow vs. broad peaks. Background in MACS’s peak-building model
- Assessment of peak qualities and reproducibility using IDR method.
- Removal of blacklisted peak regions
- Visualization (Creating bigwigs, heatmaps, histogram plots of peak distributions using DeepTools)
- Motif analysis (Homer/MEME suite, describe the position weight matrix)
- Annotation (Homer and GREAT)
- Differential peak analysis (cover briefly and connect to DESeq2/EdgeR). Usage of BedTools to create count matrix for reads.
The detailed schedule will be distributed to accepted participants prior to the start of the workshop.
Number of participants
- Participants are expected to use their own computers.
- Basic knowledge of ChIP-seq and ATAC-seq from wet-lab
- Familiarity with NGS data
- Comfortable in usage of Linux command line (Bash)
- LUNARC account
This workshop is organised by the Research School in Stem Cell Biology in collaboration with the StemTherapy Bioinformatics Core Facility. Please be aware that the number of participants is limited. Participation in the workshop is free of charge for members of the Research School in Stem Cell Biology.
WHEN AND WHERE?
March 5, 2019 at 9.00 - 17.00
Senior postdoc, Molecular Lymphopoiesis Lab
Email: Jonas [dot] Ungerback [at] med [dot] lu [dot] se
PhD student, Stem Cells and Leukemia Lab
Email: Parashar [dot] Dhapola [at] med [dot] lu [dot] se
Applications will open on November 14, 2018 and will run until December 5, 2018.
Applicants will be informed of the outcome of their application via email within one week after the application deadline.