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Flow cytometry - sorting course

Aim and format

The course aims to provide practical knowledge of droplet based cell sorting (FACS) that can be directly transferred to research questions.

Learning outcomes

On completion of the course, the participants shall be able to:

  • theoretically explain how cell sorting works

  • set up FACS instruments and conduct quality controls

  • sort on a droplet based cell sorter

  • troubleshoot the most common problems that might occur during cell sorting

  • do a risk assessment of cell sorting

Course content

The course includes the following elements:

  • the theory behind cell sorting

  • start-up, shut-down and quality control of a cell sorter

  • sorting of beads and cells to fit different types of experiments

  • troubleshooting, quality control and validation of cell sorting

  • doing a risk assessment

Course design

The course is mainly focused on practical exercises in regards to cell sorting, but does include literature studies and a few theoretical lectures. The results of these exercises are presented to, and discussed by, the course coordinator and course participants.

Lecturers

  • Anna Fossum, FACS Core Facility at Lund Stem Cell Center
  • Anna Hammarberg, MultiPark - Cellomics and Flow Cytometry Core Facility
  • Charlotta Böiers, FACS Core Facility at Lund Stem Cell Center
  • Zhi Ma, FACS Core Facility at Lund Stem Cell Center

Eligibility

  • Completion of basic training in flow cytometry (Basic course in flow cytometry or equivalent)
  • Active FACS user

Course fee

  • This course is not free of charge, and will cost 10,000 SEK.
  • Non-academia participants (e.g., companies) please contact us for pricing information. 

This course is a co-arrangement between the Research School in Stem Cell Biology, the Lund University Neuroscience Graduate School, the StemTherapy FACS Core Facility at Lund Stem Cell Center, and the MultiPark Cellomics and Flow Cytometry Core Facility. Please be aware that the number of participants is limited. Preference is given to PhD students and postdoctoral fellows who are affiliated with these networks, and to active FACS users.

 

When and Where?

November 28 - December 2, 2022

Time: 9.00 - 17.00
Venue: FACS Lab BMC B12*

Apply

Registration deadline: November 18, 2022

Applicants will be informed of the outcome of their application via email within one week after the application deadline.

*Venue and format remain subject to change dependent on the changing circumstances of COVID-19 and connected regulations. 

Syllabus MEFLCS1 

Flow Cytometry - sorting course, MEFLCS1 

1.5 credits, Third cycle

Flow Cytometry, sorting course (pdf 124,9 kB)

Contact

Charlotta Böiers
Director StemTherapy FACS Core Facility
Charlotta [dot] Boiers [at] med [dot] lu [dot] setarget="_self"

Anna Fossum
Project Manager StemTherapy FACS Core Facility
Anna [dot] Fossum [at] med [dot] lu [dot] se

Course development

Anna Fossum
FACS Core Facility at Lund Stem Cell Center

Anna Hammarberg
MultiPark - Cellomics and Flow Cytometry Core Facility

Charlotta Böiers
Director StemTherapy FACS Core Facility

Zhi Ma
FACS Core Facility at Lund Stem Cell Center