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Symposium on single cell technologies in stem cell research

Scope

The scope of this symposium is to provide a forum to discuss the development and applications of single cell approaches in cell biology. What are the underlying biological and technical reasons for conducting such experiments? In what way does data from such approaches, as opposed to more traditional studies on pools of cells, change our understanding of cellular systems and individual cell properties/behavior? What types of novel biological insights can be obtained? What are the inherent limitations, challenges and current bottlenecks for broader implementation?

We aim to approach these questions from a more educational perspective and will actively engage students and postdocs in the conversation.

Welcome!

Registration

The registration deadline is May 17.

Program

Program: Symposium on single cell technologies in stem cell research

 

Thursday, May 31, 2018

 

13.00 - 13.30

Registration


13.30 - 13.35 

Welcome note

Christine Karlsson and David Bryder, Research School in Stem Cell Biology


13.35 – 14.10 

Interrogating dopamine neuron development and diversity using single cell RNA sequencing

Thomas Perlmann, Karolinska Institute, Sweden

Chair: Sara Nolbrant (Parmar lab)


14.10 - 14.45 

Single-cell methods to define the heterogeneity of hematopietic stem cell populations

Göran Karlsson, Lund Stem Cell Center, Lund University, Sweden

Chair: Stijn Vanhee (Yuan lab)


14.45 - 15.10

Coffee break


15.10 - 15.45 

Elucidating lung stem cells and their niches at single cell resolution

Mark Krasnow, Stanford University, USA

Chair: Martina de Santis (Wagner lab)


15.45 - 16.20 

Lentiviral cellular barcoding: opportunities and limitations

Joan Yuan, Lund Stem Cell Center, Lund University, Sweden

Chair: Mikael Sommarin (G Karlsson lab)


16.20 - 16.55 

Clonal analysis of young and aged hematopoietic stem cells

Hiro Nakauchi, Stanford University, USA

Chair: Sarah Warsi (S Karlsson lab)


16.55 - 17.00

Closing remarks day 1


17.00 - 20.00

After work mingle with food and drinks

Stamstället, BMC H10

 

Friday, June 1, 2018

 

08.45 - 09.00

Registration


09.00 - 09.35 

Seeing the trees for the forest - using single cell assays in different hematopoietic research areas

Charlotta Böiers, Lund Stem Cell Center, Lund University, Sweden

Chair: Jonas Ungerbäck (Sigvardsson lab)


09.35 - 10.10 

Elucidating hematopoietic reprogramming at the single cell level

Filipe Pereira, Lund Stem Cell Center, Lund University, Sweden

Chair: Ella Quist (Ahlenius lab)


10.10 - 10.40

Coffee break


10.40 - 11.15 n

Biological and technical considerations using single-cell gene expression profiling

Anders Ståhlberg, Sahlgrenska Cancer Center, Gothenburg, Swede

Chair: Maria Jassinskaja (Hansson lab)


11.15 - 11.50 

Single cell transcriptomics for identification of disease mechanisms in human brain

Konstantin Khodosevich, Biotech Research & Innovation Centre (BRIC), Denmark

Chair: Marie Jönsson (Jakobsson lab)


11.50 - 11.55

Closing remarks


13.00 - 16.00

Break-out session: Analysing single-cell gene expression data in virtual reality

Shamit Soneji, Director Bioinformatics Core Facility, Lund Stem Cell Center, Lund University, Sweden

Venue: Bioinformatics Core Facility at BMC B12

When and where?

May 31 - June 1, 2018

Segerfalk Lecture Hall, BMC A10

Organising committee

This symposium is arranged by the Lund Research School in Stem Cell Biology.

Christine Karlsson
Christine [dot] Karlsson [at] med [dot] lu [dot] se (Christine[dot]Karlsson[at]med[dot]lu[dot]se)

Claire McKay
Claire [dot] McKay [at] med [dot] lu [dot] se (Claire[dot]McKay[at]med[dot]lu[dot]se)

David Bryder
David [dot] Bryder [at] med [dot] lu [dot] se (David[dot]Bryder[at]med[dot]lu[dot]se)

 

Single cell symposium 2018. Photo: Christine Karlsson
Single cell symposium 2018. Photo: Christine Karlsson

 

Shamit Soneji explains how to analyse single-cell gene expression data in virtual reality. Photo: Christine Karlsson
Shamit Soneji explains how to analyse single-cell gene expression data in virtual reality. Photo: Christine Karlsson

 

Marie Jönsson and Stefan Lang.
Marie Jönsson and Stefan Lang. Photo: Christine Karlsson