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UniStem workshops 2016

Can you cut into DNA? How do blood stem cells look in the microscope? What is a stem cell scientist?

These and many more questions will be answered by motivated PhD students, postdoctoral fellows, senior researchers, and science communicators during the UniStem Day's afternoon sessions on March 11th, 2016. Below you find a list of workshops, lectures, and hands-on sessions that we will offer during UniStem Day at Lund Stem Cell Center. These workshops will be held in Swedish and/or English.

Welcome!


Workshops på BMC

1. Human stem cell research: Ethical and legal aspects

Ethical issues in human embryonic stem cell research. Legal regulation in Europe regarding human embryonic stem cell research. Ethical issues in translational stem cell research. Diversity of religious approaches regarding stem cell research.

Contact

kristina [dot] hug [at] med [dot] lu [dot] se (Kristina Hug), Researcher at the Department of Medical Ethics


2. Att läsa vetenskapliga nyheter

Forskningsnyheter når oss ofta via media. Det är i dagstidningar eller på TV vi får nyheterna om de senaste försöken med till exempel stamceller. Det är också här vi får de mer fördjupande reportagen om forskningens möjligheter, men också dess risker och etiska dilemman. Som läsare, eller TV-tittare, är det centralt att kunna ha ett kritiskt perspektiv på dessa nyheter. I denna session presenterar vi och diskuterar på vilka olika sätt media förmedlar forskningsnyheter till oss. Vi läser och kritisk granskar några av de vetenskapliga nyheter som stamcellsforskarna vid Lunds universitet producerat de senaste åren.

Kontakt

kristofer [dot] hansson [at] kultur [dot] lu [dot] se (Kristofer Hansson), Forskare och docent etnologi, Institutionen för kulturvetenskaper, Ledare för forskningsgruppen The Cultural Studies Group of Neuroscience

karolina [dot] lindh [at] kultur [dot] lu [dot] se (Karolina Lindh), Forskare och FD biblioteks- och informationsvetenskap, Institutionen för kulturvetenskaper, Forskare i gruppen The Cultural Studies Group of Neuroscience


3. Behavioural assessment of neural stem cell transplants – from animal models to functional assessment

In this workshop we will present how researchers assess neural stem cells in vivo. We will give an introduction about research on laboratory animals, how we create models of neurodegenerative diseases, and how to assess the neural stem cells in vivo. The focus is on Parkinson’s disease and the transplantation of cells into the brain of rodents. After the workshop the student will have a basic idea about the animal models used for transplantation, how to assess graft survival and the immune-response, as well how a neural stem cell transplant can improve the behaviour of a rodent that has been rendered parkinsonian. The workshop will be based on presentations of the most commonly used behavioural test as well as some equipment. Due to the restricted nature of animal testing the practical part of the session will be limited.

Contact

deirdre [dot] hoban [at] med [dot] lu [dot] se (Deirdre Hoban), Postdoctoral fellow at the Division of Developmental and Regenerative Neurobiology

andi [dot] heuer [at] med [dot] lu [dot] se (Andi Heuer), Postdoctoral fellow at the Division of Developmental and Regenerative Neurobiology

michael [dot] sparrenius [at] med [dot] lu [dot] se (Michael Sparrenius), Research technician at the Division of Developmental and Regenerative Neurobiology


4. Cutting into DNA, genetic engineering approaches for science and medicine

This workshop will give you an overview on how DNA can be used in a laboratory setting in the context of research and medicine. What is the application of DNA to current medicine? How do we design and build genetic structures and systems? How is this work relevant for near and long term future medical approaches? The workshop will start with a short lecture, followed by a game/competition of putting together so called DNA plasmids for various purposes.

Contact

david [dot] yudovich [at] med [dot] lu [dot] se (David Yudovich), PhD student at the Division of Molecular Medicine and Gene Therapy

ludwig [dot] schmierer [at] med [dot] lu [dot] se (Ludwig Schmierer), PhD student at the Division of Molecular Medicine and Gene Therapy


5. Hur blir man stamcellsforskare?

En föreläsning om hur det går till att doktorera och göra karriär inom medicinsk forskning och speciellt stamcellsbiologi. Föreläsarens egen karriär kommer att användas som exempel. Föreläsningen kommer att beskriva vilka grundutbildningar man kan gå, vilka vägar det finns in i forskningen, hur doktorandtiden ser ut, vad man kan göra sedan osv. Det kommer att finnas mycket utrymme för eleverna att ställa frågor om allt möjligt angående temat och det kommer att ges endel tips och råd.

Kontakt

goran [dot] karlsson [at] med [dot] lu [dot] se (Göran Karlsson), Ledare för forskargruppen Stem Cells and Leukemia


6. Precious hematopoietic stem cells in cord blood

In the theoretical part (15 minutes) we will explain reasons to use hematopoietic stem cells (HSC) in the clinic and in research. We will focus on cord blood derived HSC. The cord connects the mother and her baby during pregnancy and after delivery can be used as a source to obtain HSCs. In practical part in the lab (30 minutes), we will explain and show major steps of HSC isolation from cord blood and how to assess the purity of isolated cells by flow cytometry.

Contact

justyna [dot] rak [at] med [dot] lu [dot] se (Justyna Rak), Postdoctoral fellow at the Division of Molecular Medicine and Gene Therapy

mehrnaz [dot] safaee_talkhoncheh [at] med [dot] lu [dot] se (Mehrnaz Safaee Talkhoncheh), PhD student at the Division of Molecular Medicine and Gene Therapy


7. Snitta hjärnan och hitta transplantat

Ni kommer att få se hur man snittar en hjärna och hur man sen märker in den med speciella antikroppar för att kunna visualisera transplanterade stamceller. I mikroskopet letar vi sen upp cellerna och tittar på hur de transplanterade cellerna ser ut.

Kontakt

malin [dot] parmar [at] med [dot] lu [dot] se (Malin Parmar), Ledare för forskargruppen Developmental and Regenerative Neurobiology

ulla [dot] jarl [at] med [dot] lu [dot] se (Ulla Jarl), Biomedicinsk analytiker i gruppen Developmental and Regenerative Neurobiology


8. Identify dead cells through flow cytometers

We would like to explain to students the general ways to distinguish dead cells, viable cells or the apoptotic cells on FACs analysis.  Students will be asked to kill cells with different methods, such as applying heat-shock, detergent or ethanol on the cells. This hand on activity will take about 15 min. The general principles will be explained in front of the instrument at BMC for another 15 min at B12 Canto room. Students will be encouraged to ask questions in another 10 min.

Contact

hooi_min [dot] tan_grahn [at] med [dot] lu [dot] se (Hooi Min Tan Grahn), Postdoctoral fellow at the Division of Molecular Medicine and Gene Therapy

anna [dot] rydstrom [at] med [dot] lu [dot] se (Anna Rydström), Research engineer at the Division of Molecular Medicine and Gene Therapy


9. Isolate stem cells with flow cytometry

Introducing students to the Flow Cytometry Sorting technique used to isolate stem cells obtaining pure populations of rare cell types. We will sort out one type of fluorescently labeled beads from many others into plates. This bead type will be at a minute concentration of 1%. We use beads to illustrate the difficulties and convenience of separating stem cells from all other cell types with this technique (20 min). Next, we move to the high content screening facility and show students automated image analysis of bulk sorted vs. purified beads (20 min).

Contact

teona [dot] roschupkina [at] med [dot] lu [dot] se (Teona Roschupkina), FACS Core Facility at Lund Stem Cell Center

zhi [dot] ma [at] med [dot] lu [dot] se (Zhi Ma), FACS Core Facility at Lund Stem Cell Center

anna [dot] hammarberg [at] med [dot] lu [dot] se (Anna Hammarberg), MultiPark - Cellomics and Flow Cytometry Core Facility


10. Hematopoietic stem cells in the bone marrow

Our white blood cells are produced in bone marrow in the adult. In our lab, part of our work is focused on studying the development of those cells using the mouse system.We will isolate hematopoietic cells from mouse bones and put them on slides (cytospin) to see the different morphologies after staining them. Using a microscope, we will look at the slides and identify blood cell progenitors as well as the different mature blood populations.

Contact

alya [dot] zriwil [at] med [dot] lu [dot] se (Alya Zriwil), PhD student at the Division of Molecular Hematology

lilian [dot] wittmann [at] med [dot] lu [dot] se (Lilian Wittmann), Research technician at the Division of Molecular Hematology


11. Passera benmärgstromaceller

Benmärgstromacellerna skapar en essentiell miljö för blodstamcellerna i kroppen. Vi studerar därför de signaler som dessa stromaceller producerar för att kunna lära oss att expandera blodstamceller utanför kroppen. Detta skulle kunna öka chanserna för blodstamcellstransplantation. Stromacellerna växer snabbt utanför kroppen och måste därför passeras innan de blir överväxta. I denna workshop kommer man att få lära sig grunderna i cellodlingskonstens ABC - passera och räkna celler.

Contact

mattias [dot] magnusson [at] med [dot] lu [dot] se (Mattias Magnusson), Ledare för forskargruppen Regulation of stem cells in normal and malignant hematopoiesis

simon [dot] hultmark [at] med [dot] lu [dot] se (Simon Hultmark), Doktorand i gruppen Regulation of stem cells in normal and malignant hematopoiesis


12. Embryonic development: paving the way for stem cell-based therapies

This workshop will include a media-supported overview of the main models in developmental biology and their role in the common effort to understand how stem cells give rise to the numerous cell types in a complex multicellular organism (15min). The students will also be introduced in a hands-on fashion to the most accessible model, and a chance to further explore their observations under the microscope (30min)

Contact

pedro [dot] rifes [at] med [dot] lu [dot] se (Pedro Rifes), Postdoctoral fellow at Human Neural Developmental Biology


13. Blood stem cells under the microscope

If you ever wondered how stem cells look like under a microscope, or at this very moment realised that you do, this workshop is for you! You will get a look at live blood stem cells, as well as mature blood cells, under the microscope.

Contact

svetlana [dot] soboleva [at] med [dot] lu [dot] se (Svetlana Soboleva), PhD student at the Division of Molecular Medicine and Gene Therapy

taha [dot] sen [at] med [dot] lu [dot] se (Taha Sen), PhD student at the Division of Molecular Medicine and Gene Therapy


14. Gene expression analysis: Are you faster than a robot?

In this workshop we will give an introduction on gene expression and how we can measure it by using qPCR. Moreover, we will introduce our pipetting robot to the students. They will then have the possibility of practicing pipetting and challenging our robot.

Contact

rebecca [dot] petri [at] med [dot] lu [dot] se (Rebecca Petri), PhD student at Molecular Neurogenetics

karolina [dot] pircs [at] med [dot] lu [dot] se (Karolina Pircs), Postdoctoral fellow at Molecular Neurogenetics


15. Direct conversion of skin cells into neurons and microscopy

This workshop will include a tour of the lab where cell culture is performed. In our lab, we reprogram skin cells from Parkinson’s disease patients into neurons. The students will be able to see the morphological changes that occur along the process in these cells, and get some insight about the procedures used for conversion (approximately 30 minutes). After that, they will have the chance to look at the cells at the stage where conversion is complete, under the fluorescence microscope (15 min).

Contact

maria [dot] pereira [at] med [dot] lu [dot] se (Maria Pereira), student at Developmental and Regenerative Neurobiology

janelle [dot] drouin-ouellet [at] med [dot] lu [dot] se (Janelle Drouin-Ouellet), Postdoctoral fellow at Developmental and Regenerative Neurobiology


Workshop på LUX

Filmvisning och Q&A med två stamcellsforskare
Q&A session där eleverna kan få ställa vilka frågor som helst kring stamceller och kring yrket stamcellsforskare. Vi kommer visa 3 filmer: "A Stem Cell Story" -  "What are embryonic stem cells?" - "What are iPS cells?"

Kontakt

agnete [dot] kirkeby [at] med [dot] lu [dot] se (Agnete Kirkeby), Ledare för forskargruppen Human Neural Development

henrik [dot] ahlenius [at] med [dot] lu [dot] se (Henrik Ahlenius), Ledare för forskargruppen Stem Cells, Aging and Neurodegeneration


Hitta hit

LUX Aula: Helgonavägen 3, 223 62 Lund

BMC: Sölvegatan 19, 223 62 Lund


 

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