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Flow cytometry - introductory course

Aim and format

The aim of this 3-day theoretical course is to give participants a working knowledge of flow cytometry covering the following aspects:

  • Basic concepts of flow cytometry
  • Instrumentation - basic principles of operation
  • Techniques - cell handling, staining, designing of protocol
  • Data acquisition and analysis
  • Applications of flow cytometry

Learning outcomes

On completion of the course, the participants shall be able to:

  • plan a flow cytometry experiment
  • account for the principles behind spillover and relate this to the need to apply compensation 
  • explain how to combine fluorochromes and select appropriate control samples.

Course content

The course includes the following elements:

  • an introduction to the basic principles of flow cytometry

  • common applications of flow cytometry

  • fluorochromes, compensation, spillover, control samples

  • planning of flow cytometry experiments.

Course design

Different forms of teaching are used including theoretical lectures, practical demonstrations, theoretical group work and individual discussion forums. The results of these exercises are presented to, and discussed by, the course coordinator and course participants.


  • Anna Fossum, FACS Core Facility at Lund Stem Cell Center
  • Anna Hammarberg, MultiPark - Cellomics and Flow Cytometry Core Facility
  • Charlotta Böiers, FACS Core Facility at Lund Stem Cell Center
  • Zhi Ma, FACS Core Facility at Lund Stem Cell Center

Course fee

  • RSSCB and Multipark members: No course fee
  • External users: 2000 SEK

This course is a co-arrangement between the Research School in Stem Cell Biology, the Lund University Neuroscience Graduate School, the StemTherapy FACS Core Facility at Lund Stem Cell Center, and the MultiPark Cellomics and Flow Cytometry Core Facility. Priority is given to PhD students, postdoctoral fellows and technical staff who are affiliated with these networks.

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When and where?

March 23-25, 2020

Time and venue: TBD

Applications will open in February 2020.

Syllabus MEFLIN1

Flow Cytometry, introductory course, MEFLIN1

1 credit, Third cycle

Flow Cytometry, introductory course (pdf 184,1 kB)


Charlotta Böiers
Operational director StemTherapy FACS Core Facility
Charlotta [dot] Boiers [at] med [dot] lu [dot] se

Christine Karlsson
Executive director Research School in Stem Cell Biology
Christine [dot] Karlsson [at] med [dot] lu [dot] se

Joan Yuan
Scientific director StemTherapy FACS Core Facility
Joan [dot] Yuan [at] med [dot] lu [dot] se