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FACS Machines

Sorters:

 

BD FACS Aria IIu SORP


The Aria IIu SORP cell sorter has 3 laser lines: violet at 405nm, blue at 488nm and red at 635nm. It can detect up to 12 colours and two scatter parameters.

For the configuration click the link: BD FACS Aria IIu SORP Filter and Mirrors

 

BD FACS Aria III
 

The BD FACS Aria III has 5 laser lines: violet at 405nm, blue at 488nm, red at 635nm, green at 561 nm and near UV at 375nm. The violet and near UV are co-linear and cannot be used simultaneously. It can detect up to 16 colours and two scatter parameters.

For the configuration click the link: BD FACS Aria III Filter and Mirrors

 

BD FACS Melody
 

The BD FACS Melody is contained within a biosafety hood which makes it suitable for sorting primary human samples and transduced cells. The Melody has three laser lines: violet at 405 nm, blue at 488 nm and yellow-green at 561 nm.  It can detect 8 colours and two scatter parameters.

For the configuration click the link: BD FACS Melody Filter and Mirrors

 

 

Picture of sorters at the FACS core facility
The BD FACS Aria IIu, Aria III and Melody cell sorters (from right to left).

 

Analysers:

 

BD LSR II
 

The BD LSR II has three laser lines: violet at 405 nm, blue at 488nm and red at 640 nm. It can detect 12 colours and two scatter parameters.

For the configuration click the link: BD FACS LSR II SORP Filter and Mirrors

 

BD LSR Fortessa
 

The BD LSR Fortessa has four laser lines: violet at 405 nm, blue at 488nm, yellow-green at 561 nm and red at 640 nm. It can detect 18 colours and two scatter parameters.

For the configuration click the link: BD FACS LSR Fortessa SORP Filters and Mirrors

 

BD LSR Fortessa X20
 

This analyser is a good supplement to our BD LSRFortessa and also has the UV laser to give more fluorochrome choices for your multi-colour analysis. The X-20 has five laser lines: UV at 375 nm, violet at 405 nm, blue at 488nm, yellow-green at 561 nm and red at 640 nm. It can detect 18 colours and two scatter parameters.

For the configuration click the link: BD LSR Fortessa X20 Filters and Mirrors

 

 

Picture of analysers at the FACS core facility
The BD LSRFortessa, LSRFortessa X-20 and the LSR II (from right to left).

 

 

FACS Machines

 

Cell Sorting Water

MACHINE SET-UP PROTOCOLS