FACS Instruments
Learn more about the cell sorters and analysers available at the FACS Core Facility
Sorters:
BD FACS Aria III
The BD FACS Aria III has 5 laser lines: near UV at 375nm, violet at 405nm, blue at 488nm, yellow-green at 561 nm and red at 635nm. The violet and near UV are co-linear and cannot be used simultaneously. It can detect up to 16 colours and two scatter parameters.
Configuration Protocol: BD FACS Aria III Filters and Mirrors
Plate Set-Up Protocol: BD FACS Aria III Plate Set-Up Protocol
BD FACS Aria IIu SORP
The Aria IIu SORP cell sorter has 3 laser lines: violet at 405nm, blue at 488nm and red at 635nm. It can detect up to 12 colours and two scatter parameters.
Configuration Protocol: BD FACS Aria IIu SORP Filters and Mirrors
Plate Set-Up Protocol: BD FACS Aria IIu SORP Plate Set-Up Protocol
BD FACS Melody
The BD FACS Melody is contained within a biosafety hood which makes it suitable for sorting primary human samples and transduced cells. The Melody has three laser lines: violet at 405 nm, blue at 488 nm and yellow-green at 561 nm. It can detect 8 colours and two scatter parameters.
Configuration Protocol: BD FACS Melody Filters and Mirrors
BD FACSymphony S6
Our most advanced and latest addition! The BD FACSymphony S6 has 5 laser lines: UV at 355nm, violet at 405nm, blue at 488nm, yellow-green at 561 nm and red at 635nm. It can detect 30 colours and two scatter parameters.
Configuration Protocol: BD FACS Symphony s6 Cell Sorter Filter Configuration
Analysers:
Cytek Aurora
The Cytek Aurora is a full-spectrum flow cytometer with 5 lasers (UV 355nm, violet 405nm, blue 488nm, yellow-green 562nm and red 640nm) that allows the simultaneous analysis of up to 64 markers on cells.
This instrument is currently located at BMC D14.
BD Symphony A1
The BD Symphony A1 has four laser lines: violet at 405 nm, blue at 488 nm, yellow-green at 561 nm and red at 637 nm. It can detect 16 colours, two scatter parameters and also has a small particle side scatter detector.
Configuration Protocol: BD Symphony A1 Filter Configuration
This instrument is available for use through generous support from the Mats Paulsson Foundation and Professor Filipe Pereira.
BD LSR Fortessa
The BD LSR Fortessa has four laser lines: violet at 405 nm, blue at 488nm, yellow-green at 561 nm and red at 640 nm. It can detect 17 colours and two scatter parameters.
Configuration Protocol: BD FACS LSR Fortessa SORP Filters and Mirrors
BD LSR Fortessa X20
This analyser is a good supplement to our BD LSRFortessa and also has the UV laser to give more fluorochrome choices for your multi-colour analysis. The X-20 has five laser lines: UV at 375 nm, violet at 405 nm, blue at 488nm, yellow-green at 561 nm and red at 640 nm. It can detect 18 colours and two scatter parameters.
Configuration Protocol: BD LSR Fortessa X20 Filters and Mirrors
