Lund Stem Cell Center Electrophysiology Facility
Welcome to the StemTherapy's Electrophysiology Core Facility!
Facility is designed to provide researchers with high quality core electrophysiology techniques and expert knowledge to investigate cells and tissues that exhibit electrical properties. The platform can be used for both, standard and tailored electrophysiological services depending on the needs of the investigator. The main set-up consists of HEKA EPC10 double-patch clamp amplifier, Olympus BX51WI microscope with multi-channel fluorescence capability and differential interference contrast for live imaging and optical manipulation of cells (e.g. optogenetics and calcium imaging) and Hamamatzu C11440-10C camera. The facility is also equipped with Multielecrode Array (MEA) system and there is a possibility to perform micromanipulator assisted collection of single cells for qPCR (Patch-Seq). The core facility is located at BMC B10 inside the cell culture laboratory making it ideal for, but not restricted to, investigation of in vitro generated and expanded cell lines and cultures.
Major applications available for the investigators include:
- Patch clamp recording in vitro (cell cultures) and ex vivo (tissue preparations) to study intrinsic electric properties of a single cell, such as resting membrane potential, action potential parameters, presence and function of various channels and receptors as well as synaptic connectivity and nature of synaptic transmission. Our main focus is neural lineage (e.g. neurons and astrocytes), but other cell types can also be recorded (e.g. cardiomyocytes, β cells)
- Optogenetic manipulation of cell function to study, e.g. synaptic profile of isolated populations of the cells in cell cultures and tissue preparations
- Optical imaging of live cells in cultures and tissue preparations to monitor intracellular Ca2+ level alterations
- Extracellular field potential recordings to study synaptic plasticity ex vivo in brain tissue preparations (predominantly in hippocampus)
- Multielectrode Array (MEA) recordings of extracellular field potentials from cell cultures and tissue preparations
Electrophysiology Core Facility
Natalia Avaliani, PhD
Phone: +46 46 2220515
Mobile: +45 25854642
natalia [dot] avaliani [at] med [dot] lu [dot] se