Flow Cytometry Sorting Course - MEFLCS1
Interested in gaining practical knowledge of droplet based cell sorting (FACS) that can be directly transferred to research questions?
Then join this new cell sorting course hosted by the Research School in Stem Cell Biology, the Lund University Neuroscience Graduate School, the StemTherapy FACS Core Facility at Lund Stem Cell Center, and the MultiPark Cellomics and Flow Cytometry Core Facility!
All are welcome to apply, please be aware that the number of participants is limited. Please be aware that the number of participants is limited. Preference is given to PhD students and postdoctoral fellows who are affiliated with these networks, and to active FACS users.
Aim and format
The course aims to provide practical knowledge of droplet based cell sorting (FACS) that can be directly transferred to research questions.
On completion of the course, the participants shall be able to:
theoretically explain how cell sorting works
set up FACS instruments and conduct quality controls
sort on a droplet based cell sorter
troubleshoot the most common problems that might occur during cell sorting
do a risk assessment of cell sorting
The course includes the following elements:
the theory behind cell sorting
start-up, shut-down and quality control of a cell sorter
sorting of beads and cells to fit different types of experiments
troubleshooting, quality control and validation of cell sorting
doing a risk assessment
The course is mainly focused on practical exercises in regards to cell sorting, but does include literature studies and a few theoretical lectures. The results of these exercises are presented to, and discussed by, the course coordinator and course participants.
- Anna Fossum, FACS Core Facility at Lund Stem Cell Center
- Anna Hammarberg, MultiPark - Cellomics and Flow Cytometry Core Facility
- Charlotta Böiers, FACS Core Facility at Lund Stem Cell Center
- Zhi Ma, FACS Core Facility at Lund Stem Cell Center
- Completion of basic training in flow cytometry (Basic course in flow cytometry or equivalent)
- Active FACS user
- Free participation for PhD students from the European Economic Area (EEA) and Switzerland.
- External participants: 4000 SEK