The platform automates a continuous loop of staining, high-resolution imaging, and gentle signal erasure. By utilizing photobleaching or specialized release mechanisms instead of harsh chemicals, the system erases fluorescent signals without damaging the delicate tissue morphology. This gentle approach allows a single sample—whether it is FFPE, frozen tissue, or an organoid—to undergo dozens of consecutive cycles, enabling true sequential multiomics by analyzing both spatial transcriptomics and proteomics on the exact same slide.
To process the massive amounts of imaging data generated by these experiments, the platform integrates with MACS iQ View software. This specialized software translates complex image pixels into actionable biological insights through automated cell segmentation, phenotypic clustering, and deep spatial microenvironment analysis.